SNAPGENE VIEWER TUTORIAL HOW TO

Rab5 from position 1330 to position 1977.To switch back to mRFPrab5 click Window in the top menu and select mRFPrab5. Right click ORF frame 2 in the map and select Edit Feature:Ĭhange name of the feature in NeoR/KanR and click OK:Ĭhange the name of ORF frame 1 and annotate it as a fusion. Change name of the feature in mRFP1-Rab5įill in the names, start and stop positions of the two genes and click OK.

SNAPGENE VIEWER TUTORIAL HOW TO

See the introduction tutorial video and the working with features tutorial video for further details on how to annotate sequences in SnapGene. Go to the Changing what you see in SnapGene tutorial to see how you can switch between views, change a view, show additional info. SnapGene is not the ideal tool for designing primers. be able to make sterilized selective liquid media for growing up one of the bacterial colonies containing the gene of interest to produce large quantities of the desired plasmid DNA.be able to isolate bacterial colonies carrying the plasmid by analyzing the agarose gel and determine samples containing the gene of interest.be able to make an agarose gel, load the gel with your PCR reaction samples, and conduct agarose gel electrophoresis of samples.be able to screen resistant colonies for the gene of interest by amplifying individual bacterial colony DNA through Restriction Enzyme Digestion.be able to plate transformed bacterial cells on selective plates and identifyresistant colonies.be able to make selective media plates for plating and growing transformed bacterial cells (antibiotic selective or X-gal).be able to perform a bacterial transformation reaction to transform competent bacterial cells with recombinant plasmid DNA.be able to recombine a digested DNA fragment and plasmid vector in a ligation reaction to make recombinant plasmid DNA carrying a gene of interest.be able to conduct a restriction digestion to prepare DNA fragments and vectors for the construction of recombinant DNA.be able to identify compatible restriction sites for cloning.It does not take into account the ability of primers to form hairpins or dimers nor does it check the specificity of the primers. This is Part A, Constructing Recombinant DNA, under the module topic, Molecular Cloning.

This topic part has two sections: Content Tutorial & Animations. Selection from the Campbell and Reece, AP Edition Biology, Seventh Edition, 2005 “Most methods for cloning pieces of DNA in the laboratory share certain general features. One common approach uses bacteria (most often, Escherichia coli) and their plasmids. Bacterial plasmids are relatively small, circular DNA molecules that replicate separate from a bacterial chromosome. For cloning genes or other pieces of DNA in the laboratory, a plasmid is first isolated from a bacterial cell, and then the foreign DNA is inserted into it. The resulting plasmid is now a recombinant DNA molecule, combining DNA from two sources. The plasmid is returned to a bacterial cell, producing a recombinant bacterium, which reproduces to form a clone of identical cells. Because the dividing bacteria replicate the recombinant plasmid and pass it on to their descendants, the foreign gene is “cloned” at the same time. Therefore the clone of cells contains multiple copies of the gene. Cloned genes are useful for two basic purposes: to make many copies of a particular gene and to produce a protein product. Researchers can isolate copies of a cloned gene from bacteria for use in basic research or to endow an organism with a new metabolic capability, such as pest resistance. For example, a resistance gene present in one crop species might be cloned and transferred into plants of another species. Alternatively, a protein with medical uses, such as human growth hormone, can be harvested in large quantities from bacterial cultures carrying the cloned gene for the protein. 385-386) Technique Overview: Cloning a Human Gene in a Bacterial Plasmid Most protein-coding genes exist in only one copy per genome-something on the order of one part per million of DNA-so the ability to clone such rare DNA fragments is extremely valuable.” (Campbell & Reece, AP Edition Biology Seventh Edition, pp.

Snapgene viewer tutorial